Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy
Article Title: Drug Delivery–mediated Control of RNA Immunostimulation
doi: 10.1038/mt.2009.147
Figure Lengend Snippet: In vitro RNA interference of influenza virus using native and chemically modified siRNAs. Cultured Vero cells were infected with PR/8 influenza virus and transfected with siRNAs. Viral titer is expressed as a percentage relative to nontreated control viral titers. The dose–response RNA interference effects of siRNA were not significantly altered by chemical modifications. The different siRNA duplexes are identified by sense and antisense strand (S/AS) as listed in . The parent unmodified siRNA is represented by 4746/4747; 4746/4747 MM represents a mismatched version of 4746/4747, while all other duplexes represent introduction of additional modifications into the original 4746/4747 parent siRNA duplex. siRNA, short-interfering RNA.
Article Snippet: Chemically modified siRNAs with the siNP-1496 sequence and GL3 firefly luciferase sequence (si1955/Luc) were synthesized by Alnylam Pharmaceuticals (Cambridge, MA).
Techniques: In Vitro, Virus, Modification, Cell Culture, Infection, Transfection, Control, Small Interfering RNA