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firefly luciferase gene sequence gl3  (Promega)

 
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    Promega firefly luciferase gene sequence gl3
    Firefly Luciferase Gene Sequence Gl3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gl3+sequence/firefly+luciferase+coding+sequence/pm24854846-67-1-5
    Average 90 stars, based on 1 article reviews
    firefly luciferase gene sequence gl3 - by Bioz Stars, 2026-11
    90/100 stars

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    Plasmid Preparation:

    Article Title: Erratum: Ultrasound-assisted non-viral gene transfer to the salivary glands
    Article Snippet: A total of 50mg of pCMV-GL3 (a GL3 Luc-expressing plasmid constructed by the insertion of a cytomegalovirus (CMV) promoter into the multiple cloning site of pGL3-basic; Promega, Madison, WI, USA) was mixed with 50mg of microbubble solution, either 100 or 15% Definity solution v/v with phosphate-buffered saline. .. For Ad-mediated gene transfer, a non-replicative Ad Serotype 5 vector based on the Stratagene AdEasy system was created by cloning the GL3 sequence (from the Promega pGL3 Basic vector) into the multiple cloning site of the pShuttle-CMV vector (Stratagene, La Jolla, CA, USA). .. The shuttle vector was recombined into the AdEasy backbone, and the resultant vector, Ad-CMVGL3, was upscaled and purified to a titer of B3.5 1012 vp ml 1.

    Cloning:

    Article Title: Erratum: Ultrasound-assisted non-viral gene transfer to the salivary glands
    Article Snippet: A total of 50mg of pCMV-GL3 (a GL3 Luc-expressing plasmid constructed by the insertion of a cytomegalovirus (CMV) promoter into the multiple cloning site of pGL3-basic; Promega, Madison, WI, USA) was mixed with 50mg of microbubble solution, either 100 or 15% Definity solution v/v with phosphate-buffered saline. .. For Ad-mediated gene transfer, a non-replicative Ad Serotype 5 vector based on the Stratagene AdEasy system was created by cloning the GL3 sequence (from the Promega pGL3 Basic vector) into the multiple cloning site of the pShuttle-CMV vector (Stratagene, La Jolla, CA, USA). .. The shuttle vector was recombined into the AdEasy backbone, and the resultant vector, Ad-CMVGL3, was upscaled and purified to a titer of B3.5 1012 vp ml 1.

    Sequencing:

    Article Title: Erratum: Ultrasound-assisted non-viral gene transfer to the salivary glands
    Article Snippet: A total of 50mg of pCMV-GL3 (a GL3 Luc-expressing plasmid constructed by the insertion of a cytomegalovirus (CMV) promoter into the multiple cloning site of pGL3-basic; Promega, Madison, WI, USA) was mixed with 50mg of microbubble solution, either 100 or 15% Definity solution v/v with phosphate-buffered saline. .. For Ad-mediated gene transfer, a non-replicative Ad Serotype 5 vector based on the Stratagene AdEasy system was created by cloning the GL3 sequence (from the Promega pGL3 Basic vector) into the multiple cloning site of the pShuttle-CMV vector (Stratagene, La Jolla, CA, USA). .. The shuttle vector was recombined into the AdEasy backbone, and the resultant vector, Ad-CMVGL3, was upscaled and purified to a titer of B3.5 1012 vp ml 1.



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    In vitro RNA interference of influenza virus using native and chemically modified siRNAs. Cultured Vero cells were infected with PR/8 influenza virus and transfected with siRNAs. Viral titer is expressed as a percentage relative to nontreated control viral titers. The dose–response RNA interference effects of siRNA were not significantly altered by chemical modifications. The different siRNA duplexes are identified by sense and antisense strand (S/AS) as listed in . The parent unmodified siRNA is represented by 4746/4747; 4746/4747 MM represents a mismatched version of 4746/4747, while all other duplexes represent introduction of additional modifications into the original 4746/4747 parent siRNA duplex. siRNA, short-interfering RNA.

    Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

    Article Title: Drug Delivery–mediated Control of RNA Immunostimulation

    doi: 10.1038/mt.2009.147

    Figure Lengend Snippet: In vitro RNA interference of influenza virus using native and chemically modified siRNAs. Cultured Vero cells were infected with PR/8 influenza virus and transfected with siRNAs. Viral titer is expressed as a percentage relative to nontreated control viral titers. The dose–response RNA interference effects of siRNA were not significantly altered by chemical modifications. The different siRNA duplexes are identified by sense and antisense strand (S/AS) as listed in . The parent unmodified siRNA is represented by 4746/4747; 4746/4747 MM represents a mismatched version of 4746/4747, while all other duplexes represent introduction of additional modifications into the original 4746/4747 parent siRNA duplex. siRNA, short-interfering RNA.

    Article Snippet: Chemically modified siRNAs with the siNP-1496 sequence and GL3 firefly luciferase sequence (si1955/Luc) were synthesized by Alnylam Pharmaceuticals (Cambridge, MA).

    Techniques: In Vitro, Virus, Modification, Cell Culture, Infection, Transfection, Control, Small Interfering RNA